Description
The H5N1 (avian flu) hemagglutinin ELISA Pair Set is for the quantitative determination of H5N1 (avian flu) hemagglutinin. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for H5N1 (avian flu) hemagglutinin coated on a 96-well plate. Standards and samples are added to the wells, and any H5N1 (avian flu) hemagglutinin present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-H5N1 (avian flu) hemagglutinin monoclonal antibody is then added, producing an antibody-antigen-antibody ""sandwich"". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of H5N1 (avian flu) hemagglutinin present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm